7

7. Alteración de la especificidad y de la velocidad de catálisis enzimática. Producción de dominios de proteínas. Aumento de la solubilidad de las proteínas recombinantes.

Bibliografía:

Engineering nitrile hydratase activity into a cysteine protease by a single mutation. E Dufour, AC Storer, R Menard Biochem (1995) 34, 16382-16388

L. Abrahmsén, J. Tom, J. Burnier, K.A. Butcher, A. Kossiakoff, J.A. Wells (1991) Biochemistry 30, 4151-4159.(subtiligasa)

D.Y. Jackson, J. Burnier, C. Quan, M. Stanley, J. Tom, J.A. Wells. (1994) Science 266, 243-247

Single-chain antigen-binding proteins. (1988). RE bird, KD Hardman, JW Jacobson, S Johnson, BM Kaufman, SM Lee, T Lee, SH Pope, GS Riordan, M Whitlow. Science,

Redesign of the coenzyme specificity of a dehydrogenase by protein engineering. N.S. Scrutton, A. Berry & R. N. Perham. Nature 343, 38-43 (1990).

Improving protein solubility through rationally designed amino acid replacements: solubilization of the trimethoprim-resistant type S1 dihydrofolate reductase. GE Dale, C Broger, H Langen, A D;Arcy, & D Stüber. Prot. Eng. 7, 933-939 (1994)

Current Opinion in Structural Biology (COSB) No 4 (Engineering and design) de los volúmenes 1-9.