6

6. Introducción de sitios de unión, sitios de corte o sitios de marcaje.

Bibliografía:

ALGUN PROTOCOLO DE PURIFICACION CON COLAS DE HISTIDINA

Protein design: novel metal-binding sites. L. Reagan. Trends Biochem. Sci.(1995) 20: 280-285.

Structure of an engineered, metal-actuated switch in trypsin. M.E. McGrath, B.L. Haymore, N.L. Summers, C.S. Craik, R.J. Fletterick. Biochemistry (1993), 32, 1914-1919.

Regulation of serine protease activity by an engineered metal switch. J.N. Higaki, B.L. Haymore, S. Chen, R.J. Fletterick, C.S. Craik. (1990). Biochemistry 29, 8582-8586.

Regulation of proteolytic activity by engineered tridentate metal binding loops. S Halfon, CS Craik, J. Am. Chem. Soc. (1996) 118: 1227-1228.

Reversible permeabilization of plama membrenes with an engineered switchable pore. MJ Russo, H Bayley, &M Toner. (1997) Nat. Biotechnol. (1997) 15: 278-282.

A 2.2 Å resolution crystal structure of a designed zinc finger protein bound to DNA. Nat. Struct. Biol. (1996) 3: 940-945.

Killing HIV-infected cells by transduction with an HIV protease-activated caspase-3 protein. A.M. Vocero-Akbani, N.V. Heyden, N.A. Lissy, L. Ratner, S.F. Dowdy, Nature Medicine (1999) 5:29-33.

Disection of an enzyme by protein engineering. The N- and C-terminal fragments of barnase form a native-like complex with restored enzymic activity. Sancho, J. and Fersht, A.R. J. Mol. Biol. 224, 741-747 (1992)

ALGUN PROTOCOLO DE PURIFICACION DE PROTEINAS DE FUSION CON ROTURA CON ALGUN FACTOR DE COAGULACION O SIMILAR

Thiol reactive probes. en Handbook of fluorescent probes and research chemicals cap. 2, pp 47-62. Molecular ProbesTM.

Current Opinion in Structural Biology (COSB) No 4 (Engineering and design) de los volúmenes 1-8.