5. La inactivación irreversible. Estrategias y ejemplos de estabilización frente a reacciones irreversibles.

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• Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation. D. A. Estell, T. P. Graycar, & Wells, J.A. J. Biol. Chem. 260, 6518-6521 (1985).

• Identification and elimination by site-directed mutagenesis of thermolabile aspartyl bond in Aspergillus awamori glucoamylase. H-M Chen, C. Ford, & P.J. Reilly. Prot. Eng. 8, 575-583 (1995)

• Stabilization of lysozyme against irreversible inactivation by alterations of the Asp-Gly sequences.. H. Tomizawa, H. Yamada, Y. Hashimoto, & T. Imoto. Prot Eng. 8, 1023-1028 (1995)

• Bacillus lentus alkaline proteease with increased stability. T. Christianson, D. Goddette, B.F. Ladin, M.R. Lau, C. Paech, B.B. Reynolds, C.R. Wilson, S-S Yang. US Patent 1994, 5: 340-735.

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