12

12. Estrategias y ejemplos de diseños de novo: felix, betabelina, ´coiled-coils'.

Bibliografía:

Characterization of a helical protein designed from first principles. Regan, L. & De Grado, W.F. Science 241: 976-978 (1988)

Crystal structure of a1: implications for protein design:. Hill, C.P., Anderson, D.H., Wesson, L., De Grado, W.F. & Eisemberg, D. Science 249: 543-546 (1990)

De novo design, expression, and characterization of Felix: a four-helix bundle protein of native-like sequence. Hecht, M.H., Richardson, J.S., Richardson, D.C. & Ogden, R.C. Science 249: 884-891 (1990)

a-helical coiled coils and bundles: how to design an a-helical protein. Cohen, C. & Parry, A.D. Proteins:structure, function, and genetics 7: 1-15 (1990)

Engineering of betabellin 14D: Disulfide-induced folding of a ß-sheet protein. Yan, Y. & Erickson, B.W. Protein Science 3, 1069-1093 (1994).

Characteristics of a de novo designed protein. Tanaka, T., Kimura, H., Hayashi, M., Fujiyoshi, Y., Fukuhara, K-I & Nakamura, H. Protein Science 3, 419-427 (1994).

Crystal structure of a synthetic triple-stranded a-helical bundle. Lovejoy, B., Choe, S, Cascio, D., McRorie, D.K., DeGrado, W.F., Eisenberg, D. (1993). Science 259, 1288-1293.

A synthetic peptide ligase. Severin, K., Lee, D.H., Kennan, A.J., Ghadiri, M.R. (1997). Nature 389, 706-709.

A swicht between two-, three-, and four-stranded coiled colis in GCN4 lucine zipper mutants. Harbury, P.B., Zhang, T., Kim, P.S., Alber, T. (1993). Science, 262, 1401-1407.

Design of a monomeric 23-residue polypeptide with defined tertiary structure. Struthers, M.D., Cheng, R.P. & Imperiali, B. Science, 271, 342-345.